CovidV2, Main, Exploration, bibRecord, 001362

Alternative Molecular Tests for Virological Diagnosis

Identifieur interne : 001362 ( Main/Exploration ); précédent : 001361; suivant : 001363

Alternative Molecular Tests for Virological Diagnosis

Auteurs : Francesca Sidoti [Italie] ; Massimiliano Bergallo [Italie] ; Cristina Costa [Italie] ; Rossana Cavallo [Italie]

Source :

Molecular Biotechnology [ 1073-6085 ] ; 2013-03-01.

RBID : ISTEX:1B6B090E047177E4BA527E13ECC6F0DC35B04A77

English descriptors

KwdEn :
- HDA, Isothermal amplification, LAMP, NASBA, Virological diagnosis.

Abstract

Abstract: Several nucleic acid amplification techniques (NAATs), particularly PCR and real-time PCR, are currently used in the routine clinical laboratories. Such approaches have allowed rapid diagnosis with a high degree of sensitivity and specificity. However, conventional PCR methods have several intrinsic disadvantages such as the requirement for temperature cycling apparatus, and sophisticated and costly analytical equipments. Therefore, amplification at a constant temperature is an attractive alternative method to avoid these requirements. A new generation of isothermal amplification techniques are gaining a wide popularity as diagnostic tools due to their simple operation, rapid reaction and easy detection. The main isothermal methods reviewed here include loop-mediated isothermal amplification, nucleic acid sequence-based amplification, and helicase-dependent amplification. In this review, design criteria, potential of amplification, and application of these alternative molecular tests will be discussed and compared to conventional NAATs.

Url:

DOI: 10.1007/s12033-012-9533-8

Affiliations:

Le document en format XML

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Alternative Molecular Tests for Virological Diagnosis

Alternative Molecular Tests for Virological Diagnosis

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration Covid (26 mars)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.